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Figure 3 | Respiratory Research

Figure 3

From: Pneumocystis murina colonization in immunocompetent surfactant protein A deficient mice following environmental exposure

Figure 3

Transmission of P. murina infection from immunocompetent SP-A deficient (KO) mice to severe combined immunodeficiency ( SCID ) mice. SCID mice were housed in the same cage with immunosuppressed (i/s) (black triangles) or immunocompetent (i/c) (inverted black triangles) KO seed mice for two weeks. A third group of SCID mice was not exposed to any KO mice as a negative control (open squares). Transmission of Pneumocystis infection by direct exposure to an animal infected with Pneumocystis has been referred to as "seeding" and the infected mice are referred to as "seeds". The SCID mice were sacrificed, RNA extracted from the lungs and cDNA synthesized. P. murina large mitochondrial ribosomal RNA gene mRNA levels were quantitated in the samples by qPCR. The data are the mean ± the SEM and are expressed as P. murina nuclei per reaction. *p < 0.05 i/s seed vs. i/c seed by t test. Each group contained at least 5 mice.

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