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Figure 4 | Respiratory Research

Figure 4

From: Loss of a single N-linked glycan from the hemagglutinin of influenza virus is associated with resistance to collectins and increased virulence in mice

Figure 4

Loss of a N-linked glycosylation site from the HA of S and Mo clones of Beij/89. (A) HI titres of anti-HA monocloncal antibodies against L, S and Mo clones of Beij/89. HI titres are expressed as the recipricol of the highest dilution of ascites fluid to inhibit 4 hemagglutinating units (HAU) of virus. Data presented are the mean of 3 independent experiments and are representative of results obtained with 3-5 independent clones of L, S or Mo viruses. * = p < 0.01, Kruskal-Wallis test. (B) Immunoblot showing the HA proteins of plaque-purified L, S and Mo clones of Beij/89. Proteins from purified viruses were resolved by SDS-PAGE under non-reducing conditions on a 5-10% gel and transferred to a nictrocellulose membrane. To detect the HA glycoproteins, the blot was incubated with anti-HA mAb CY3/3 and developed, as described in Materials and Methods. The location of the HAO monomer at approximately 80 kDa is indicated with an arrow.

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Respiratory Research

ISSN: 1465-993X

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