Figure 1
From: Hypoxia regulates human lung fibroblast proliferation via p53-dependent and -independent pathways
Effect of hypoxia and DFX on NHLF proliferation. A; Effects of hypoxia and DFX on BrdU incorporation in cultured NHLF: Cultured NHLF were exposed to 0.1% or 2% oxygen, or 100 μM DFX, in the presence of BrdU for 24 h. Severe hypoxia (0.1% oxygen) and DFX suppressed BrdU incorporation, but moderate hypoxia (2% oxygen) significantly enhanced incorporation. Data are expressed as mean ± SE (n = 6). *P < 0.05 versus the 21% oxygen control. B; Cell cycle analysis of NHLF exposed to hypoxia and DFX: Cultured NHLF (synchronized at G0/1 phases by serum depletion) were exposed to 0.1% or 2% oxygen, or 100 μM DFX, in the presence of 10% FBS. After 24 h, the cells were harvested and cell cycle analyses were performed using a flow cytometer with propidium iodide staining. Severe hypoxia and DFX significantly decreased the percentage of cells in the S plus G2/M phases, whereas moderate hypoxia had the opposite effect. Representative histograms are shown and the bar graph shows data expressed as mean ± SE (n = 4). Open bars, percentages of cells in the G0/1 phases; solid bars, percentages of cells in the S + G2/M phases. *P < 0.05 versus the 21% oxygen control.