Figure 5
Cellular LDH release and IL-8 protein after particle exposure. A: LDH release after 24 h incubation with different concentrations of 1 μm and 0.05 μm particles. An exposure concentration of 6 × 109 particles per cell culture well significantly increases the LDH release vs. negative control (NC) (* = p < 0.01). B: IL-8 protein after 24 h particle exposure. The concentration of 6 × 109 1 μm particles induces a significant IL-8 secretion compared to the negative control (NC) (* = p < 0.01). A positive control (PC) was generated with TNFα stimulation. At no other of the tested exposure concentrations was a significant LDH release or IL-8 secretion observed.