Figure 6
From: Dysregulated apoptosis and NFκB expression in COPD subjects
NF[kappa]B analysis in induced sputum. (a) Identification of granulocyte nuclei (gated area shown) in sputum by comparing separated granulocyte nuclei from sputum sample (left) with whole sputum nuclei (right) (b) Sputum granulocyte nuclei: FL-3-peak versus FL-3 integral dot plot showing a singlet gate to exclude aggregates (c) Percentage of neutrophils expressing p50 and p65 (NFκB activation) in induced sputum in non-smokers (NS), healthy smokers (HS) and in COPD subjects. In COPD subjects closed symbols = current smokers and open symbols = ex-smokers. Mann-Whitney U test was used to determine any significant differences. COPD n = 12; HS n = 6; NS n = 8. Line represents median value.