Dysregulated apoptosis and NFκB expression in COPD subjects

Respiratory Research

Table 3 Comparison of apoptotic assays from sputum neutrophils

	Method 1	Method 2			Method 3
	% Apoptotic neutrophils ¹	% Av ⁺ /PI ^-	% Av ⁺ /PI ⁺	% Av ^- /PI ⁺	% Tunel +ve cells ²
ALL SUBJECTS	10.9 (1.22)	17.19 (2.85)	37.63 (5.27)	21.67 (4.02)	28.56 (4.09)

Method 1: Microscopic morphological identification. Method 2: Annexin V/Propidium iodide (PI) staining. Method 3: Identification of DNA fragmentation (Tunel assay).
% AV⁺/PI^-: early apoptosis, % AV^+/PI⁺: later stage apoptosis/secondary necrosis and % AV^-/PI⁺: necrosis.
¹Significantly different compared to Tunel +ve cells (p < 0.001), % AV⁺/PI⁺ (p < 0.001) and % AV^-/PI⁺ neutrophils using Tukey's multiple comparison post-hoc test. ²Significantly different compared to % AV⁺/PI^- cells (p < 0.05).
Data are expressed as mean (SEM)

ISSN: 1465-993X