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Figure 2 | Respiratory Research

Figure 2

From: Ascorbic acid pre-treated quartz stimulates TNF-α release in RAW 264.7 murine macrophages through ROS production and membrane lipid peroxidation

Figure 2

RT-PCR of TNF-α mRNA in quartz-treated RAW 264.7 cells. TNF-α mRNA transcription was monitored in RAW 264.7 macrophages by RT-PCR analysis from 0.5 to 24 h following cell stimulation with 100 μg/ml of QA (white bars) or Q particles (black bars). Results are the mean of three independent experiments performed in triplicate, and are expressed as TNF-α mRNA, normalized on the GAPDH transcription, relative to control cells at time-zero. The asterisks indicate a significant difference between samples challenged with Q and QA particles (T Test, p < 0.025).

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