Figure 4

A) Representative RT-PCR gel of VEGF isoforms in ATII cells. Lanes are as follows: (1–4) human ATII cell samples, (5) blank, (6) negative control, (7–9) sequenced positive controls for VEGF₁₂₁, VEGF₁₆₅ and VEGF₁₈₉ respectively, (10) 100 kb ladder (bottom marker denotes 100 bp, top marker denotes 300 bp). B-D): Semiquantitative densitometry data (n = 8, in triplicate) showing VEGF₁₂₁, VEGF₁₆₅and VEGF₁₈₉ in response to LPS (10 μg/ml), TNF-α (10 ng/ml), IL-1β, VEGF₁₆₅ (0.1,1,10 ng/ml) at 4 hours. Data are plotted as mean with bars denoting standard error. *P < 0.05 (ANOVA post hoc Bonferroni) 10 μg/ml LPS versus control (all isoforms) and 10 ng/ml VEGF₁₆₅ versus control (VEGF₁₆₅ only). ^#P < 0.01 (ANOVA, post hoc Bonferroni) 10 ng/ml VEGF versus control (VEGF₁₂₁ and VEGF₁₈₉). E-F): Further real-time PCR analysis confirmed the effect of LPS and VEGF on VEGF₁₆₅ and VEGF₁₈₉ (*p < 0.05 fold change compared to control).