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Figure 3 | Respiratory Research

Figure 3

From: Sildenafil attenuates pulmonary inflammation and fibrin deposition, mortality and right ventricular hypertrophy in neonatal hyperoxic lung injury

Figure 3

Paraffin lung sections stained with polyclonal anti-vWF antibody (panels A-C) to visualize the endothelium of pulmonary vessels for the quantification of pulmonary vessel density (panel D) of room-air (RA, panel A) and O 2 -exposed controls (panel B), and age-matched pups treated with sildenafil (100 mg/kg/day) under hyperoxia (panel C) at 10 days of age. Pictures were taken at a 200× magnification. Arrows in panels A-C indicate vWF-positive blood vessels. Quantification of pulmonary vessel density (panel D), mean linear intercept (panel E), alveolar septum thickness (panel F) and medial wall thickness (panel J) in room air-exposed littermates (white bars), O2-exposed control pups (black bars) and 100 mg/kg/day sildenafil-treated pups (Sil100, gray bars). Paraffin lung sections stained with monoclonal anti-ASMA antibody for the visualization of medial wall thickness in pulmonary arterioles (panels G-I) of room-air (RA, panel G) and O2-exposed controls (panel H), and age-matched pups treated with sildenafil (100 mg/kg/day) under hyperoxia (panel I) at 10 days of age. Pictures were taken at a 1000× magnification. The enlargements shown in the lower right parts of panels A, B and C are indicated in the boxed areas. Values are expressed as mean ± SEM in at least 6 different rat pups per group. a = alveolus **p < 0.01 and ***p < 0.001 versus age-matched O2-exposed controls. ΔΔΔ p < 0.001 versus room air-exposed controls.

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