Figure 2
From: Arginine deficiency augments inflammatory mediator production by airway epithelial cells in vitro
Hyper-responsive IL-6 (A, C) and IL-8 (B, D) production by NCI-H292 cells maintained in MEM without arginine. Cells were plated and, after overnight culture, exposed for 20 h to fresh medium (MEM plus L-glutamine and arginine (MEM-A+) or MEM with L-glutamine only (MEM-A-)) to a concentration range of TNF-α (A, B) and LPS (C, D). Typical experiment is shown out of three experiments (triplicate samples; mean ± SEM). *P < 0.05, **P < 0.01 and ***P < 0.001 as compared to MEM-A+.