Figure 7
From: Arginine deficiency augments inflammatory mediator production by airway epithelial cells in vitro
Modulation of IL-6 (A) and IL-8 (B) promoter-driven transcription by LPS and poly-L-arginine. Cells were transfected with an IL-6 or IL-8 promoter-driven chloramphenicol acetyltransferase (CAT)-expressing vector. Transfected cells were exposed to normal medium (Med), medium containing 5 μg/ml lipopolysaccharide (LPS), medium containing 5 μg/ml poly-L-arginine (PLA) or medium containing LPS plus PLA (LPS+PLA) for 18 h, respectively. The production of CAT was measured by ELISA. Mean ± SD of three experiments are shown. **P < 0.01 and ***P < 0.001 as compared to Med, #P < 0.05 and ###P < 0.001 as compared to LPS+PLA.