Figure 3

Lgl1 protein is reduced in Lgl1 ^+/- mice. Lgl1 immunohistochemistry (N ≥ 4, all groups) was performed as described in Methods and quantified with Northern Eclipse as per Nadeau et al. 2006 [3]. Representative images are shown. Modest to moderate differences in Lgl1 protein levels were observed between Lgl1 ^+/+ and Lgl ^+/- mice at ages PN1, 7 and 14 (Arrows indicate the reduction of Lgl1 at tips of alveolar septa in Lgl1 ^+/- mice at PN 14). At PN28, there was considerable variability between Lgl1 ^+/- mouse lung samples. In some cases, Lgl1 protein appeared to be reduced, consistent with the observed reduction in Lgl1 mRNA (shown) while in others, effects on protein expression were not detectible. Magnification: 400×