Figure 5

Lgl1 ^+/- mice display reduced resistance and elastance in response to MCh challenge. Airway hyperresponsiveness (AHR) in response to increasing doses of aerosolized MCh was assessed in 4 week old Lgl1 ^+/+ and Lgl1 ^+/- mice (N = 13 and 9 respectively). Response was measured as maximal resistance (A) and elastance (B). Means are presented ± SEMs. A. Lgl1 ^+/- mice displayed similar resistance to their wild type littermates at baseline. At doses of MCh ranging from 25 to 50 mg/ml Lgl1 ^+/- mice showed reduced resistance compared to wild type littermates. (p ≤ 0.05). B. Lgl1 ^+/- mice displayed similar elastance to their wild type littermates at baseline. In the presence of 50 mg/ml MCh, Lgl1 ^+/- mice displayed significantly less elastance than their wild type littermates (p ≤ 0.05). C. Tropoelastin mRNA isolated from total lungs of Lgl1 ^+/+ and Lgl1 ^+/- mice was quantified by quantitative real-time RT PCR (N ≥ 4). A significant reduction in tropoelastin mRNA was observed at E14.5 in the Lgl1 ^+/- mice. From E16.5 until PN1 tropoelastin mRNA levels in lungs of Lgl1 ^+/- mice were similar to those observed in wild type littermates (p < 0.05). At PN28, tropoelastin mRNA levels were significantly elevated relative to controls (p < 0.01).