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Figure 7 | Respiratory Research

Figure 7

From: Inflammatory cytokines, goblet cell hyperplasia and altered lung mechanics in Lgl1 +/- mice

Figure 7

Postnatal Lgl1 +/- mice display goblet cell hyperplasia and increased expression of MUC5AC. A. MUC5AC was quantified in mRNA isolated from total lungs of Lgl1 +/+ and Lgl1 +/- mice by quantitative real-time RT PCR (N ≥ 5). A significant increase in MUC5AC mRNA was observed at PN7 and PN14 in the Lgl1 +/- mice (p < 0.05). B. Lung sections of Lgl1 +/+ and Lgl1 +/- mice were stained with Period Acid Schiff (PAS) stain to visualize the goblet cells. Representative images are shown.PAS positive cells were rarely seen in wild type mice during the early post natal period, however a considerable number of PAS positive cells were observed in both the trachea and upper bronchi of the Lgl1 +/- mice during this same time period. Magnification: 400× C. Scatter plot illustrating bimodal distribution of goblet cells in heterozygous Lgl1 +/- mice (significant difference between subgroups, p = 0.017, Mann Whitney U test).

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