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Figure 5 | Respiratory Research

Figure 5

From: PKA and Epac cooperate to augment bradykinin-induced interleukin-8 release from human airway smooth muscle cells

Figure 5

Role of Epac and PKA in GTP-loading of Rap1 and Rap2. hTERT-airway smooth muscle cells were fractioned as described in Material and Methods. Expression of membrane-associated or cytosolic Rap1 (A) and Rap2 (B) was evaluated and normalized to the content of the cell fraction-specific marker proteins caveolin-1 and β-actin, respectively. hTERT-airway smooth muscle cells were stimulated for 10 min without (Control) and with 100 μM 8-pCPT-2'-O-Me-cAMP or 500 μM 6-Bnz-cAMP. Thereafter, GTP-loaded and total Rap1 (A) or Rap2 (B) were determined as described in Material and Methods. Representative immunoblots are shown above with the respective densitometric quantifications underneath them (n = 3-5). *P < 0.05, **P < 0.01 compared to unstimulated control.

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