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Figure 2 | Respiratory Research

Figure 2

From: NO2 inhalation induces maturation of pulmonary CD11c+ cells that promote antigen-specific CD4+ T cell polarization

Figure 2

Effects of depleting CD11c + cells during sensitization in an NO 2 -promoted allergic asthma model. Eight- to fifteen-week-old female CD11c-DTR Tg+ and Tg- mice were administered 4 ng DT/g of body weight via i.p. injection on day -1. All mice then underwent inhalation of 15 ppm NO2 for 1 hour followed by 30 minutes of aerosolized ova on day 0. All mice were challenged with aerosolized ova on days 14, 15, and 16. Differential cell counts were measured from the BAL fluid 48 hours after the final ova challenge (A). Values shown are mean ± SEM with 8 Tg- and 5 Tg+ mice per group. CD4+ cells were isolated from spleens by positive selection on day 18 and co-cultured with antigen presenting cells (APCs) from naïve C57BL/6J mice and 100 μg/ml ova. Conditioned medium was collected at 96 hours and analyzed for the Th2 cytokines IL-4 (B), IL-5 (C), and IL-13 (D), and the Th17 cytokine IL-17 (E) by ELISA. No cytokines were detected in medium from APCs cultured alone or from CD4+ T cells cultured with APCs in the absence of ova. Values shown are mean ± SEM with 4-5 mice per group. The ova-specific immunoglobulins IgE (F) and IgG1 (G) were measured from serum collected 48 hours after the final ova challenge (day 18) by ELISA using serum from Alum/ova-immunized mice to generate standard curves. Values shown are mean ± SEM with 7-10 mice per group. Statistics were computed by unpaired Student's t test. * denotes p < 0.05 and ** denotes p < 0.01 compared with respective Tg- samples.

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