Figure 4
Kinin B 1 (B1R) and B 2 (B2R) receptor mRNA expression. Tracheal segments were cultured for 4 days in presence of vehicle (DMSO, control) or nicotine (Nic, 10 μM) (A). JNK inhibitor SP600125 or PDE4 inhibitor YM976 was added to 4-day culture with nicotine (10 μM) (B). Each data point is derived from 3-6 experiments and data is presented as mean ± S.E.M. Statistical analysis was performed using unpaired student's t-test with Welch's correction. Control vs Nic (A); Nic vs Nic+SP600125/YM976 (B). ** P < 0.01; *** P < 0.001.