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Table 2 Effects of nicotine on 5-HT- and acetylcholine-induced airway contractions

From: Nicotine enhances murine airway contractile responses to kinin receptor agonists via activation of JNK- and PDE4-related intracellular pathways

Incubation time

Nicotine (μM)

 

5-HT

 

Acetylcholine

  

n

E max (mN)

pEC 50

n

E max (mN)

pEC 50

Day 1

0 (Ctrl)

9

1.87 ± 0.32

6.47 ± 0.13

8

5.81 ± 0.74

6.51 ± 0.12

 

10

10

1.97 ± 0.26

6.45 ± 0.10

8

6.20 ± 0.62

6.46 ± 0.07

Day 2

0 (Ctrl)

11

2.01 ± 0.29

6.83 ± 0.09

8

6.45 ± 0.70

6.57 ± 0.06

 

10

12

1.99 ± 0.31

6.87 ± 0.09

8

5.95 ± 0.73

6.56 ± 0.10

Day 4

0 (Ctrl)

10

2.01 ± 0.23

6.98 ± 0.08

6

6.04 ± 1.05

6.43 ± 0.07

 

1

9

1.89 ± 0.28

7.00 ± 0.13

6

5.24 ± 0.64

6.56 ± 0.12

 

10

8

1.88 ± 0.18

6.89 ± 0.18

6

5.70 ± 0.49

6.61 ± 0.11

  1. Tracheal segments were cultured for 1, 2 or 4 days in presence of vehicle (0.1% DMSO, Ctrl) or nicotine (1 or 10 μM). Emax and pEC50 for 5-HT and acetylcholine are presented as mean ± S.E.M. Statistical analysis was performed using unpaired student's t-test with Welch's correction. Nicotine vs Ctrl (DMSO). No significant differences were found between the two groups. n = number of experiments performed.
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Respiratory Research

ISSN: 1465-993X

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