Effect of neutrophil elastase and its inhibitor EPI-hNE4 on transepithelial sodium transport across normal and cystic fibrosis human nasal epithelial cells

Table 3 Comparison of the effects of aprotinin and hNE on I _sc in control and CF HNEC.

	Change in I _scμA/cm²(% baselineI _sc)
	Aprotinin	hNE
Control HNEC	-15.7 ± 3.56 (-27.6 ± 3.47%)	9.5 ± 1.80 (18.5 ± 3.33%)
CF HNEC	-33.9 ± 5.20 * (-54 ± 8.18%) **	20.4 ± 4.47 (32.1 ± 6.7%)

Human nasal epithelial cells (HNEC) from control and CF patients were grown for 14 to 21 days on semi-permeable transwell filters until transepithelial resistance developed. Transwell filters were mounted in Ussing chamber for I _sc measurements, and exposed apically to vehicle, aprotinin (50 μg/ml), or aprotinin (for 90 min) followed by hNE (33 μg/ml). The change in I _scinduced by aprotinin represents the difference between the value of I _scat the end of aprotinin exposure and baseline I _scat the moment of aprotinin addition. The change in I _scinduced by hNE represents the difference between peak I _scvalue following hNE addition and I _scvalue at the moment of hNE addition. Results are expressed in μA/cm² and in percentage of baseline I _sc, and represent means ± SE of 4 to 10 filters for each condition. *, **: significantly different from corresponding value in control HNEC group (P < 0.05 and P < 0.01).

ISSN: 1465-993X