Figure 2
From: Phosphodiesterase 6 subunits are expressed and altered in idiopathic pulmonary fibrosis
PDE6 immunoreactivity in lung tissues from donors and IPF patients. (A) Immunoblotting was used to assess PDE6A (~105 kDa), PDE6B (~105 kDa), PDE6D (~17 kDa) and PDE6G/H (~11 kDa) expression in lung tissue homogenates from donors (n = 6) and IPF patients (n = 6). GAPDH (~37 kDa) served as a loading control. (B) Corresponding densitometric analysis normalized to GAPDH expression, white square donor and black square IPF lungs. Data were present as mean ± S.E, *P < 0.01 versus donor for PDE6D protein expression and *P < 0.001 versus donor for PDE6G/H protein expression. (C) Demonstration of PDE6A and PDE6B antibodies specificity by an antigen (peptide/protein) blocking technique. GAPDH served as a loading control. (D) Corresponding densitometric analysis normalized to GAPDH expression, BP (blocking peptide), white square without BP and black square with BP. (E) Immunoblotting showing PDE6A and PDE6B immunoreactivity in pig retina and human lung.