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Figure 6 | Respiratory Research

Figure 6

From: Smoking-mediated up-regulation of GAD67 expression in the human airway epithelium

Figure 6

Western analysis of GAD67 protein expression in small airway epithelium of healthy nonsmokers and healthy smokers. A. Upper panel - GAD67 protein expression in nonsmokers (lanes 1-6), smokers (lanes 7-12) and positive control (lane 13). Lower panel - same gel probed with anti β-actin antibody; 20 μg protein loaded per well. B. Ratio of GAD67 to β-actin. The ratio of GAD67 to β-actin is represented on the ordinate for smoker and nonsmoker bands. Error bars represent the standard error. Note in panel A, the variability in relative up-regulation of GAD67 in the smokers, similar to that observed at the mRNA level and with immunohistochemistry.

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