Figure 7From: Oxidative modification of albumin in the parenchymal lung tissue of current smokers with chronic obstructive pulmonary disease Western blot analysis of HSA and oxidised HSA degradation in human lung tissue. Human lung tissue (n = 12) was cultured with 200 μg/ml HSA or oxidised HSA and incubated for 1, 2, 4, 6, or 24 hours. Supernatants were separated on a 12% SDS-polyacrylamide gel and analysed for HSA expression using immunoblot. The supernatants cultured with HSA are depicted in Figure 7a and the supernatants cultured with oxidised HSA are shown in figure 7b. The blot depicted is a typical example of the molecular profile of HSA observed for all individuals in the study.Back to article page