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Figure 6 | Respiratory Research

Figure 6

From: Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes

Figure 6

TLR-ligands induce differential cytokine production. Bronchoalveolar cells (BAC) and monocytes (MN) were stimulated with 5 mg/mL DNA from M.tb H37 Rv, 100 ng/mL LPS and 1 ng/mL Pam3Cys for 20 hours. Cytokines were determined in culture supernatants by ELISA for TNF-α and IL-6 (A, C) and Cytokine Bead Array for IL-1β IL-10 and IL-12 (B, D and E). Culture medium and human DNA (5 mg/mL) were used as negative stimulation controls. Mean cytokine pg/mL ± SE of seven independent experiments are depicted. Statistically significant differences (p < 0.05): (*) comparing stimulated vs. controls (medium) within respective cell groups, (++) comparing bronchoalveolar cells with monocytes.

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