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Figure 10 | Respiratory Research

Figure 10

From: Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis

Figure 10

Cholesterol synthesis and HMG-CoAR mRNA expression in CF epithelial cells. (A) Cholesterol synthesis decreases by 20% in resveratrol treated 9HTEo-pCEPR (CF-model) epithelial cells compared to vehicle (DMSO) treated cells (0.8 +/- 0.03 fold below untreated cells *p-value <0.01). Mevastatin treated CF epithelial cells show a similar decrease in cholesterol synthesis compared to vehicle treated CF epithelial cells (0.78 +/- 0.05, *p-value 0.04). Data are the mean of three separate experiments. (B) HMG-CoAR mRNA expression in resveratrol (RSV) treated 9HTEo-pCEPR CF-model epithelial cells. An increase in HMG-CoAR mRNA levels is seen with resveratrol (50 μm, 24 h) treatment (2.6 +/- 0.6 fold over untreated cells, *p-value 0.03). Mevastatin (MEV) used as a positive control shows a similar increase in HMG-CoAR expression (3.172 +/- 0.1 fold over untreated cells, *p-value 0.04). Data are the mean of three separate experiments. Error bars are SEM. Mann-Whitney rank sum test used where normality test failed.

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