Figure 1From: Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesisElectrochemical determination of membrane cholesterol content from mouse nasal epithelium. A, C) Representative traces of membrane cholesterol determination in excised nasal epithelium from Cftr R117H/R117H (R/R) mice and Cftr ΔF508/ΔF508 (ΔF/ΔF) mice, respectively, compared to sibling Cftr +/+ (wt) mice. B, D) Quantification of responses between Cftr R/R and sibling Cftr +/+ (wt) nasal tissue and Cftr ΔF/ΔF nasal tissue compared to Cftr +/+ (wt) siblings. Responses are reported relative to wt response (response ratio) to indicate the fold-increase in response. Error bars represent SEM, n = 4 for each. Significance determined by t test. *p < 0.001. E) Representative traces of wt mice from the ΔF508 (ΔF) and R117H colonies. Mean response for wt mice in the ΔF and R117H colonies are 54.5 +/- 0.5 pC and 55.7 +/- 1.5 pC, respectively.Back to article page