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Figure 1 | Respiratory Research

Figure 1

From: Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis

Figure 1

Electrochemical determination of membrane cholesterol content from mouse nasal epithelium. A, C) Representative traces of membrane cholesterol determination in excised nasal epithelium from Cftr R117H/R117H (R/R) mice and Cftr ΔF508/ΔF508 (ΔF/ΔF) mice, respectively, compared to sibling Cftr +/+ (wt) mice. B, D) Quantification of responses between Cftr R/R and sibling Cftr +/+ (wt) nasal tissue and Cftr ΔF/ΔF nasal tissue compared to Cftr +/+ (wt) siblings. Responses are reported relative to wt response (response ratio) to indicate the fold-increase in response. Error bars represent SEM, n = 4 for each. Significance determined by t test. *p < 0.001. E) Representative traces of wt mice from the ΔF508 (ΔF) and R117H colonies. Mean response for wt mice in the ΔF and R117H colonies are 54.5 +/- 0.5 pC and 55.7 +/- 1.5 pC, respectively.

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