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Figure 4 | Respiratory Research

Figure 4

From: Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis

Figure 4

Specificity of CFTR inhibition in regulating membrane cholesterol content. A) Representative traces of membrane cholesterol determination in 9/HTEo-pCEPR (CF) cells after treatment with the CFTR inhibitor CFTRinh-172 for 24 h (red line) or cells with no treatment (NT, black line). B) Quantification of responses between pCEPR (CF) cells with no treatment (NT) and cells with acute (24 h) addition of the CFTR inhibitor CFTRinh-172 (20 μM). Responses are reported relative to NT response (response ratio) to indicate the fold-difference in response. Error bars represent SEM, n = 4 for each. Significance determined by t test. No significant difference was found. C) Representative traces of membrane cholesterol determination in 9/HTEo-pCEP (wt) cells after treatment with Inactive-CFTRinh-172 for 24 h (red line) or cells with no treatment (NT, black line). D) Quantification of responses between pCEP (wt) cells with no treatment (NT) and cells with acute (24 h) addition of the Inactive-CFTRinh-172 (20 μM). Responses are reported relative to NT response (response ratio) to indicate the fold-difference in response. Error bars represent SEM, n = 4 for each. Significance determined by t test. No significant difference was found.

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