Figure 7
De novocholesterol synthesis in CF mouse lung compared to matched controls. A) Deuterium incorporation into newly synthesized tissue cholesterol was measured by GC/MS. Data is normalized to each tissue wt control (represented by dotted line) and reported as fold increase of % newly synthesized cholesterol/8 h. Filled bars represent ΔF/ΔF mice and open bars represent R/R mice. The number of replicates is shown in parenthesis above each bar and represents individual assay on multiple tissue samples obtained over 3 experiments. *p < 0.05, #p < 0.01. B) Increased SRE response in CFTRinh-172 treated control epithelial cells. 9/HTEo-pCEP (wt) cells were incubated in serum free conditions for 24 h with or without 20 μM CFTRinh-172 (INH-172) in serum free media for an additional 24 h. 9/HTEo-pCEP are open bars. Data are normalized to serum free NT control levels over 3 experiments. Number (n) of samples is in parenthesis above each bar. Significance was determined by t test. Error bars represent SEM. * p < 0.0001.