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Figure 8 | Respiratory Research

Figure 8

From: Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis

Figure 8

Role of cholesterol synthesis in modulating membrane cholesterol content. (A) Membrane cholesterol content, measured as current (pA, picoamps), is decreased in resveratrol (RSV) treated 9HTEo-pCEPR (CF) epithelial cells. Control experiment is done using bare Pt on untreated cells. (B) Resveratrol treated CF epithelial cells show decreased membrane cholesterol content (0.06 pA +/- 0.01 *p-value <0.001) compared to untreated cells. Data are shown as mean of four separate experiments. (C and D) Mevastatin (MEV) treated 9HTEo-pCEPR (CF) epithelial cells show a similar significant decrease in membrane cholesterol content (0.07 pA +/- 0.01 *p-value <0.001). Data are shown as mean of four separate experiments. Error bars are SEM in all experiments. (E) Resveratrol's ability to decrease membrane cholesterol content is blocked in the presence of the AMPKi. Current measured as picoamps (pA). (F) AMPKi increases membrane cholesterol content above baseline compared to resveratrol treated cells (1.07 +/- 0.09, *p < 0.001). Untreated CF cells normalized to one. Data are from three separate experiments. Error bar is SEM.

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Respiratory Research

ISSN: 1465-993X

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