Figure 2

GC frass-induced experimental allergic asthma in C57/BL6 wild type and PAR-2-deficient mice. Wild type and PAR-2-deficient mice were sensitized on day 0 and 7 with an intraperitoneal injection of 100 ug/ml PBS or GC frass with alum (10 μg/ml). On days 14 and 19, an intratracheal inhalation was performed using PBS (40 μl) or GC frass (40 μg). On day 22, mice were anesthetized acetylcholine was injected after establishment of a stable airway pressure. In all cases the data are expressed as mean ± SEM and represent 9-10 mice per group and statistical significance determined by ANOVA. A. AHR was measured as airway pressure time index (APTI) in cm-H₂O × sec ^-1 (compared to PBS *p < 0.001 and ** = 0.016). B. Serum IgE levels were analyzed by ELISA (compared to PBS *p < 0.001, **p = 0.002). C+D. Lungs from the mice were excised, and cells dissociated and maintained in a single suspension culture for 3 days in the presence of Con A (10 μg/ml). Supernatants were removed and ELISAs were run. C. IL-5 levels (compared to PBS *p < 0.001). D. IL-13 levels (compared to PBS *p < 0.001).