Skip to main content
Figure 7 | Respiratory Research

Figure 7

From: Inhibition of IFN-γ-dependent antiviral airway epithelial defense by cigarette smoke

Figure 7

GSH monoethyl ester inhibits cigarette smoke effects on type II interferon-induced responses. A: ICAM-1 cell surface protein levels were determined using an enzyme-linked immunoassay with hTBE cells that were first treated in media without or with GSH-MEE for 2 hours. Cells were then incubated in media containing the antioxidant without or with CSE at the indicated concentrations for 4 hours, followed by incubation for 20 hours with the same compounds without or with IFN-γ. Values are expressed as mean ± S.D. (n = 3 replicates) and a significant difference (p < 0.01) in comparable CSE-treated levels between cells not incubated versus incubated with GSH-MEE is indicated by an asterisk. B: Tyrosine-701 phosphorylated and total Stat1, ICAM-1, and β-actin protein levels were assessed using immunoblot analysis of extracts from hTBE cells that were first treated in media without or with GSH-MEE for 2 hours. Cells were then incubated in media containing the antioxidant without or with CSE at the indicated concentrations for 4 hours, followed by incubation for 8 hours with the same compounds without or with IFN-γ.

Back to article page