Figure 3

PCBG induced IL-8 expression requires NF-κB and AP-1 activation. 1HAEo^- cells were transiently transfected with the IL-8 promoter (WT), the IL-8 promoter mutated at the NF-κB site (mut kB) or the IL-8 promoter mutated at the AP-1 site (mut AP-1). TK-renilla (10 ng) was co-transfected as an internal control as indicated in material and methods. Eighteen hours later, transfected 1HAEo^- cells were challenged with 100 ug/ml of PCBG. After an additional eight hours of incubation, the cells were harvested and luciferase activities were measured. The IL-8 activity was normalized to Renilla luciferase activity (relative lights units). Data were analyzed with one-way ANOVA and posttest Bonferroni comparison (***denotes p < 0.01). The data shown is the average of two independent experiments.