Figure 7

NF-κB activation is impaired in the presence of MAPKs inhibitors and an intra-calcium chelator, but not in the presence of JNK inhibitor. 1HAEo^- cells were transiently transfected with the NF-κB reporter (50 ng) and TK-renilla (10 ng) as indicated in the Material and Methods. Eighteen hours later, transfected 1HAEo^- cells were challenged with 100 μg/ml of PCBG, prior stimulation the cells were preincubated for 1 h with the different inhibitors. Eight hours later, the cells were harvested and luciferase activities were measured. The NF-κB activity was normalized to Renilla luciferase activity (relative lights units). Data were analyzed with one-way ANOVA and posttest Bonferroni comparison (*denotes p < 0.05; **denotes p < 0.01). The data shown is representative of three independent experiments.