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Figure 1 | Respiratory Research

Figure 1

From: Gene expression profiling following NRF2 and KEAP1 siRNA knockdown in human lung fibroblasts identifies CCL11/Eotaxin-1 as a novel NRF2 regulated gene

Figure 1

Changes in mRNA expression following NRF2 and KEAP1 gene-specific siRNA transfection in NHLFs. (A,C-E) Cells were transfected with negative control siRNA targeting firefly luciferase (FFL), and 2 pools of 10 siRNAs (KEAP1 pool 1 and pools 2) or an esiRNA preparation targeting KEAP1 (esiRNA KEAP1) and evaluated for KEAP1, MRP2/ABCC-2, HMOX1 and NQO1 mRNA expression by QPCR. (B-E) Cells were transfected with negative control siRNA targeting firefly luciferase (FFL), and 3 pools of 10 siRNAs targeting NRF2 (NRF2 pool 1, pool 2, pool 3) and evaluated for NRF2, MRP2/ABCC-2, HMOX1 and NQO1 mRNA expression by QPCR. Data is expressed as expression levels relative to the FFL transfection. n=4 for each data point. A student’s t-test was performed on each data set relative to the FFL data (*p < 0.5, **p < 0.01). Data are represented as mean ± standard deviation.

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