Figure 2

K-means clustering of gene signature modulated by either KEAP1 or NRF2 siRNAs. (A) K-means clustering of gene signature modulated by either KEAP1 or NRF2 siRNAs at 48 hours post-transfection. Three replicates were profiled for each siRNA pool. One way ANOVA (NRF2 or KEAP1 siRNA-transfected vs. mock-transfected samples) was performed to identify genes up- or down-regulated by NRF2 or KEAP1 siRNA at p ≤ 0.01. Data from all three replicates of each siRNA pool were combined in silico and further filtered by absolute fold change ≥ 1.15. (B) K-means clustering of the anti-correlated gene signature modulated by KEAP1 and NRF2 siRNAs. The gene signature was obtained by combining anti-correlated genes modulated by KEAP1 and NRF2 siRNAs at 30 hours (308 genes at replicates combined p<=0.05) and 48 hours (893 genes at replicates combined p<=0.05). We further removed a 43 interferon-inducible gene set resulting from a KEAP1-3 siRNA pool to obtain a final signature gene set of 1,045 sequences with 361 sequences down-regulated by NRF2 siRNAs and 684 sequences down-regulated by KEAP1 siRNAs.