Figure 8
Co-localization of HIMF with neutrophils. Paraffin-embedded lung sections from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline-challenged, and Ova-challenged mice were dual stained with rabbit anti-HIMF polyclonal antibody that was visualized by DyLight 488-conjugated goat anti-rabbit IgG antibody (green; B, G), and rat anti-neutrophil (NIMP-R14) monoclonal antibody (neutrophils) that was visualized by DyLight 594-conjugated donkey anti-rat IgG antibody (red; C, H). Cell nuclei were counterstained with DAPI (blue; A, F). The arrows in the merged images indicate co-localization of HIMF and neutrophil marker (NIMP-R14; D, I). Scale bar: 50 μm. (E, J) Merged images enlarged to show co-localization.