Figure 5

The effect of STIM1 silencing on hypoxia-induced nuclear translocation of NFATc3 in PASMCs. NFATc3 staining was visualised by confocal microscopy and immunofluorescence. The primary antibody against NFATc3 was detected using a FITC-conjugated AffiniPure goat anti-rabbit IgG secondary antibody (green). The slides were counterstained with the nuclear dye DAPI (blue). (A): Immunofluorescence image of NFATc3 in PASMCs: PASMCs were either left untreated under normoxic conditions (N), left untreated under hypoxic conditions (HCON), treated with nontargeted siRNA under hypoxic conditions (HsiNT), or treated with siRNA targeted to STIM1 under hypoxic conditions (HsiSTIM1). Representative Western blot images (B) and summarized data (C) for NFATc3 nuclear levels, Lamin B1 was used as a loading control. *:P < 0.05 compared with the Control group and #:P < 0.05 compared with the HCON and HsiNT group. The results are expressed as the mean ± SEM of three separate experiments.