Skip to main content
Figure 2 | Respiratory Research

Figure 2

From: Human leukocyte antigen-G expression in differentiated human airway epithelial cells: lack of modulation by Th2-associated cytokines

Figure 2

Presence of soluble HLA-G5 and membrane-bound HLA-G1 in dAEC by confocal microscopy. Cells were labeled using the 5A6G7 antibody for G5 (A - E) or the 01G antibody for G1 (F - J) (green in each image), and nuclei were counter-stained with Hoechst 33258 (blue). The negative control cell line JAR (A,F) and positive control cell line JEG-3 (B,G) are shown. Epithelial cells were also labeled for cytokeratin 5 (C,H), MUC5AC (D,I) or β-tubulin (E,J) (red in each image). Yellow label represents the overlap of both G5/G1 and the cell subtype marker. Background control fluorescence for each color in each image was subtracted. Images representative of 3 experiments. Bar in (E), 10 μm for all images.

Back to article page