Figure 3

Expression of HLA-G after treatment with IL-4, IL-5 or IL-13 treatment as demonstrated by qPCR. dAEC were treated with 0.1 - 10 ng/ml of each cytokine, or 10,000 U/ml of IFN-β as a positive control, for 24 hr, after which RNA expression was examined by qPCR using G5-specific primers. For experiments in which cells were treated with a Th2-associated cytokine, expression of eotaxin-3 was measured as a positive control of response. N = 6 experiments each for IL-13 and IL-4, 4 experiments for IL-5 and 8 experiments for IFN-β. *, P < 0.05 vs no treatment.