Figure 6

Intracellular detection of IL-17A and IFN-γ in lung CD4+ T cells from WT mice 2 days after elastase administration. (A) Percentages of IL-17A+ and IFN-γ+ T cells following saline treatment (Saline group) or PPE treatment (PPE group). Intracellular IL-17A and IFN-γ were assessed as described in Methods. The data shown are representative of two independent experiments. (B) The numbers of IL-17A+ and IFN-γ+ CD4 T cells in the lung of WT mice. The numbers of IL-17A+ CD4 T cells and IFN-γ+ CD4 T cells were calculated as described in Methods. Groups are the same as in (A). Data represent the mean ± SEM (n=6 in each group). *p<0.05 versus saline-treated group.