Figure 5

AEC and SAEC wound repair stimulation by individual hMSC paracrine factors. (A) Recombinant human plasma Fibronectin did not enhance AEC wound in serum-free condition (open bars) but required 0.2% FBS supplementation (black bars) (n = 6; **p < 0.01, ***p < 0.001) (B) Recombinant human plasma Fibronectin significantly increased SAEC wound repair at 100 ng/ml concentration in SF-SABM (grey bar) vs. negative control (NC) (black bar) (n = 6; *p < 0.05). (C) Recombinant human Lumican did not enhance AEC wound in serum-free condition (open bars) but required 0.2% FBS supplementation (black bars) (n = 9; **p < 0.01, ***p < 0.001). (D) Recombinant human Lumican significantly increased SAEC wound repair (n = 6; **p < 0.01, ***p < 0.001). (E) Recombinant human Periostin enhanced AEC wound repair (n = 10, **p < 0.01, ***p < 0.001; ^$p < 0.001 vs corresponding serum-free NC). (F) Periostin increased SAEC wound repair at 1 μg/ml in SF-SABM (n = 6; *p < 0.05). (G) Human recombinant IGFBP-7 did not stimulate AEC wound repair in serum-free (open bars) or 0.2% FBS supplemented conditions (black bars) (n = 9; *p < 0.001 vs 0.2% FBS supplemented NC, ^$p < 0.001 vs serum-free NC). (H) Recombinant human IGFBP-7 significantly stimulated SAEC wound repair (n = 6; **p < 0.01, ***p < 0.001). NC (negative control) represents wound repair with DMEM for AEC and serum-free SABM for SAEC. PC (positive control) represents wound repair with 10% FBS supplemented DMEM for AEC and complete SAGM for SAEC. Data presented as mean ± SD. ns = not significant. Scale bar, 200 μm.