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Figure 7 | Respiratory Research

Figure 7

From: Combined exposure to cigarette smoke and nontypeable Haemophilus influenzae drives development of a COPD phenotype in mice

Figure 7

Expression of mucin genes and goblet cell metaplasia in CS/HK-NTHi-exposed mice following RV-infection. CS/HK-NTHi-exposed mice were infected with RV or equal volume of sham and sacrificed 4 days later. (A to C) Total lung RNA was isolated, reverse transcribed and subjected to qPCR using gene specific primers and expressed as fold change over house-keeping gene, G3PDH. Data in E to G represents mean ± SD calculated from 5–6 mice per group (* different from sham infected mice, p ≤ 0.05, unpaired t test). (D and E) Paraffin lung sections from sham- or RV-infected animals were stained with PAS. Arrows point to PAS positive goblet cells. Images are representative of 3 animals per group.

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