Figure 1

Loss of TRIP-1 in human lung fetal fibroblasts enhances collagen contraction ability and induces expression of alpha-smooth muscle actin ($\mathbf{\alpha }$-SMA). A: Collagen contraction assays using HLF-F transfected with control siRNA (left) or TRIP-1 siRNA (right) at 7 days; B: % area contracted respect to original area is shown based on analysis of images like the ones shown in A) with means ± SE shown (**P < 0.05, n = 6); C: analysis of α-SMA expression in HLF-F cells transfected with control or TRIP-1 siRNA (100 nM); cells were transfected and 48 h later lysates were made and analyzed by western blot for TRIP-1, α-SMA and α-Tubulin (as a loading control); D: normalized values of TRIP-1 expression (black boxes) or α-SMA expression (hatched boxes) in control siRNA (left) or TRIP-1 siRNA (right) transfected cells, with means ± SE shown (**P < 0.05, n = 3); E) analysis of α-SMA mRNA expression by real-time PCR in control siRNA or TRIP-1 siRNA. Values are normalized to GAPDH mRNA. Experiments were performed at least three times and one representative experiment is shown, with means ± SE shown (**P < 0.05, n = 3).