Figure 7From: Pseudomonas aeruginosa elastase causes transient disruption of tight junctions and downregulation of PAR-2 in human nasal epithelial cellsImmunocytochemistrical finding and Real-time PCR analysis. (A) Immunocytochemistrical staining for occludin and claudin-1 and occludin in hTERT-transfected HNECs pretreated with 10–200 μM PAR-2 agonist before treatment with 0.1 U Pseudomonas aeruginosa elastase for 1 h. Bar: 10 μm. (B) Real-time PCR for occludin and claudin-1 mRNAs in hTERT-transfected HNECs pretreated with 100 μM PAR-2 agonist before treatment with 0.1 U Pseudomonas aeruginosa elastase for 1 h. PE: Pseudomonas aeruginosa elastase. PAR-2A: PAR-2 agonist.Back to article page