Table 1 Primers for RT-PCR
Gene | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|
G3PDH | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA | 452 |
PAR-1 | GGATATTTGACCAGCTCCTGG | AGATGGCCAGACAAGTGAAGG | 400 |
PAR-2 | CTGCATCTGTCCTCACTGGAA | ATTGCCAGGGAGATGCCAATG | 400 |
Claudin-1 | GCTGCTGGGTTTCATCCTG | CACATAGTCTTTCCCACTAGAAG | 619 |
Claudin-4 | AGCCTTCCAGGTCCTCAACT | AGCAGCGAGTAGAAG | 249 |
Claudin-7 | AGGCATAATTTTCATCGTGG | GAGTTGGACTTAGGGTAAGAGCG | 252 |
Occludin | TCAGGGAATATCCACCTATCACTTCAG | CATCAGCAGCAGCCATGTACTCTTCAC | 189 |
Tricellulin | AGGCAGCTCGGAGACATAGA | TCACAGGGTATTTTGCCACA | 200 |