Figure 4

Rosiglitazone, but not chloroquine, isoproterenol or albuterol, inhibits the initiation and development of methacholine-induced contraction. Lung slices were perfused with increasing concentrations of methacholine (MCh) in the presence of A) chloroquine (CQ) (control: n = 8, 10 μM: n = 8, 100 μM: n = 3), B) rosiglitazone (RGZ) (vehicle: n = 4, 10 μM: n = 3, 30 μM: n = 4, 100 μM: n = 4), C) isoproterenol (ISO) (control: n = 4, 10 μM: n = 4) or D) albuterol (ALB) (control n = 4, 10 μM, n = 4). Responses (mean ± S.E.M) represent the airway lumen area (normalized to the initial area), averaged over the last minute of perfusion at each MCh concentration. *p < 0.05, 2 way ANOVA, Bonferroni post-hoc.