Figure 5

Rosiglitazone and chloroquine maintain potency and efficacy despite β-adrenoceptor desensitization. Slices were incubated overnight with vehicle (0.1% water) or albuterol (ALB, 100 μM). Airways were then pre-contracted with 100 nM methacholine (MCh) prior to addition of 10 μM ALB and concentration response curves to rosiglitazone (RGZ) or chloroquine (CQ). A) Representative traces illustrating RGZ concentration-response curves following overnight incubation with vehicle or ALB. Relaxation responses to B) C) 10 μM ALB and CQ (n = 4/group) and D) E) 10 μM ALB and RGZ (n = 4/group) following overnight incubation with vehicle or 100 μM ALB. *p < 0.05, unpaired t-test.