| n | % Relaxation |
---|
| | Inhibitor alone | Inhibitor + CQ |
---|
Vehicle | 8 | 1.2 ± 2.1 | 88.1 ± 4.9 |
TEA (1 mM) | 4 | 36.3 ± 7.8*** | 39.8 ± 3.2^^^ |
Glibenclamide (1 μM) | 4 | 0.6 ± 2.4 | 89.3 ± 2.9 |
Apamin (500 nM) | 4 | 0.1 ± 2.5 | 92.8 ± 3.7 |
Charybdotoxin (10 nM) | 4 | 5.2 ± 2.1 | 83.4 ± 11.1 |
- Airways were pre-contracted with 100nM MCh prior to perfusion for 5 min in the presence of inhibitor alone, followed by inhibitor and chloroquine (10 μM). Responses (mean ± S.E.M) represent the% relaxation of the pre-contraction to MCh, averaged over the last minute of perfusion. ***p < 0.0001 cf vehicle. ^^^p < 0.0001 cf CQ alone.