Figure 10

Vegf165 requires PKC activation to maintain explant growth under hyperoxia. A’. E11.5 whole lung explants were exposed to 3% ± GO6983 (5 μM) (A-B) and 50% oxygen (C) for 3 days in the presence of Vegf165 (100 ng/ml, D), Vegf165 + DMSO (E), Vegf165 + GO6983 (5 μM, F) followed by quantification of total number of terminal branches (G) and total branch length (H). n =5 separate experiments. *p <0.05 vs. C, D, E and F. **p <0.05 vs. C and F at E11.5 + 3. Bar = 1 mm. Photographs at 4x magnification. B’ E15.5 lung post caval lobe explants (B’ A-F) were exposed to 3% ± GO6983 (5 μM) (A-B) and 50% oxygen (C) for 2 days in the presence of Vegf165 (100 ng/ml, D), Vegf165 + DMSO (E), Vegf165 + GO6983 (5 μM, F) followed by quantification of total number of terminal branches (G) and total branch length (H). n =4 separate experiments. *p <0.05 vs. C and F. **p <0.05 vs. C and F at E15.5 + 2. Bar = 0.5 mm. Photographs at 4× magnification.