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Figure 9 | Respiratory Research

Figure 9

From: Heparin binding VEGF isoforms attenuate hyperoxic embryonic lung growth retardation via a FLK1-neuropilin-1-PKC dependent pathway

Figure 9

Neuropilin-1 is required for Vegf165 activation of PKC and AKT signaling. Lysates of E11.5 + 2 (A-D) and E15.5 + 2 (E-F) lung explants exposed to 50% oxygen ± isotype control ± anti-NRP1 (10 micrograms/ml) ± Vegf165 (100 ng/ml, 15 minutes) were western blotted for pFLK1, phospho-ERK (pERK), phospho-AKT (pAKT), AKT, pan-phospho-PKC (pPKC) and PKC with actin as loading control. Representative immunoblots are shown in (A, B, C, D, E and F). Accompanying graph of n =4 experiments. *p <0.05 50% oxygen + Vegf165 vs. 50% + isotype (for pFLK1 and pPKC) and 50% oxygen + anti-NRP1 and then Vegf165 (for pFLK1, pAKT and pPKC).

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