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Figure 4 | Respiratory Research

Figure 4

From: JNK suppresses pulmonary fibroblast elastogenesis during alveolar development

Figure 4

JNK is activated in lipofibroblasts during alveolar development. (A) By lineage tracing of lung mesenchymal cells, pJNK (purple) was located within mesenchymal cells (green), and not epithelial cells (red). 20× image. (B) An enlarged area of the confocal image with Z-plane sectioning showed that individual mesenchymal cells were located entirely within the green-stained mesenchymal cells. Scale bar = 5 μm. (C-E) Monochromatic images with z-planes demonstrated the presence of pJNK within mesenchymal cells. (F) Fibroblasts constitute only a subset of lung mesenchymal cells. Co-staining for pJNK and vimentin demonstrated that pJNK was localized to vimentin-positive cells although many vimentin positive cells did not contain pJNK. 40× image. (G) Lung myofibroblast were identified by staining for α-smooth muscle actin (αSMA). pJNK did not localize with αSMA positive cells. 40× image. (H) Lung lipofibroblasts were identified by Oil-Red-O staining. Using immunofluorescent imaging and differential interference contrast microscopy pJNK was located within interstitial cells having increased numbers lipid droplets but not within lipid-containing cells in airspaces (i.e. macrophages, upper right). 40× image, Scale Bar = 50 μm. Images are representative of sections from at least three different animals.

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